Abstract
9-aminoacridine Inhibition of HIV-1 Tat Dependent Transcription
Virology journal, v 6(1), pp 114-114
24 Jul 2009
PMID: 19630958
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
As part of a continued search for more efficient anti-HIV-1 drugs, we are focusing on the possibility that small molecules could efficiently inhibit HIV-1 replication through the restoration of p53 and p21WAF1 functions, which are inactivated by HIV-1 infection. Here we describe the molecular mechanism of 9-aminoacridine (9AA) mediated HIV-1 inhibition. 9AA treatment resulted in inhibition of HIV LTR transcription in a specific manner that was highly dependent on the presence and location of the amino moiety. Importantly, virus replication was found to be inhibited in HIV-1 infected cell lines by 9AA in a dose-dependent manner without inhibiting cellular proliferation or inducing cell death. 9AA inhibited viral replication in both p53 wildtype and p53 mutant cells, indicating that there is another p53 independent factor that was critical for HIV inhibition. p21WAF1 is an ideal candidate as p21WAF1 levels were increased in both p53 wildtype and p53 mutant cells, and p21WAF1 was found to be phosphorylated at S146, an event previously shown to increase its stability. Furthermore, we observed p21WAF1 in complex with cyclin T1 and cdk9
in vitro
, suggesting a direct role of p21WAF1 in HIV transcription inhibition. Finally, 9AA treatment resulted in loss of cdk9 from the viral promoter, providing one possible mechanism of transcriptional inhibition. Thus, 9AA treatment was highly efficient at reactivating the p53 – p21WAF1 pathway and consequently inhibiting HIV replication and transcription.
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Details
- Title
- 9-aminoacridine Inhibition of HIV-1 Tat Dependent Transcription
- Creators
- Irene Guendel - George Washington UniversityLawrence Carpio - George Washington UniversityRebecca Easley - George Washington UniversityRachel Van Duyne - George Washington UniversityWilliam Coley - George Washington UniversityEmmanuel Agbottah - George Washington UniversityCynthia Dowd - George Washington UniversityFatah Kashanchi - George Washington UniversityKylene Kehn-Hall - George Washington University
- Publication Details
- Virology journal, v 6(1), pp 114-114
- Publisher
- BioMed Central
- Number of pages
- 1
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000269328400001
- Scopus ID
- 2-s2.0-69049119261
- Other Identifier
- 991021903313304721
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- Web of Science research areas
- Virology