Abstract
Abstract C003: Outer membrane vesicles from Bacteroides fragilis contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells
Cancer research (Chicago, Ill.), v 84(22_Supplement), pp C003-C003
17 Nov 2024
Abstract
Alterations to the community structure and function of the microbiome are associated with changes to host physiology, including immune responses. However, the contribution of microbial-derived RNAs carried by outer membrane vesicles (OMVs) to host immune responses remains unclear. This paper investigates the role of OMVs and OMV-associated small RNA (sRNA) species from pathogenic and commensal Bacteroides fragilis (ETBF and NTBF respectively) in eliciting different immune responses in intestinal epithelial cells. To distinguish the differences in the sRNA profiles of both strains and their OMVs, small RNA-seq was conducted, and identified enrichment of discrete sRNA species in OMVs that were also differentially expressed between the two strains. To understand the effects of OMVs on pattern recognition receptors, Toll-like receptor (TLR) reporter cells were treated with OMVs, and activation of TLRs 2, 3, 4, and 7 were observed. Treatment of Caco-2 and HT29-MTX cells with OMVs demonstrated increased expression of IL-8. Surprisingly, we discovered that degradation of RNase-accessible RNAs within ETBF OMVs, but not NTBF OMVs, resulted in vesicles with enhanced capacity to stimulate IL-8 expression, indicating that these extravesicular RNAs exert an immunosuppressive effect. This suggests a dual role for OMV-associated RNAs in modulating host immune responses, with implications for both bacterial pathogenesis and therapeutic applications.
Citation Format: Aadil Sheikh, Colin Scano, Julia Xu, Tolulope Ojo, Jessica M. Conforti, Kayla L. Haberman, Bryan King, James Lotter, Alysia Martinez, Harry Ojeas, Michelle Pujol, Juli Watkins, Emily Lin, Bernd Zechmann, Amanda Sevcik, Christie Sayes, Elyssia S Gallagher, Joshua Mell, Garth Ehrlich, Joseph H Taube, K. Leigh Greathouse. Outer membrane vesicles from Bacteroides fragilis contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells [abstract]. In: Proceedings of the AACR Special Conference in Cancer Research: Tumor-body Interactions: The Roles of Micro- and Macroenvironment in Cancer; 2024 Nov 17-20; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2024;84(22_Suppl):Abstract nr C003.
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Details
- Title
- Abstract C003: Outer membrane vesicles from Bacteroides fragilis contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells
- Creators
- Aadil Sheikh - Baylor UniversityColin Scano - Baylor UniversityJulia XuTolulope Ojo - Baylor UniversityJessica M. Conforti - Baylor UniversityKayla L. Haberman - Baylor UniversityBryan King - Baylor UniversityJames Lotter - Baylor UniversityAlysia MartinezHarry Ojeas - Baylor UniversityMichelle PujolJuli WatkinsEmily Lin - Baylor UniversityBernd Zechmann - Baylor UniversityAmanda Sevcik - Baylor UniversityChristie Sayes - Baylor UniversityElyssia S Gallagher - Baylor UniversityJoshua Mell - Philadelphia UniversityGarth Ehrlich - Philadelphia UniversityJoseph H Taube - Baylor UniversityK. Leigh Greathouse - Baylor University
- Publication Details
- Cancer research (Chicago, Ill.), v 84(22_Supplement), pp C003-C003
- Publisher
- AMER ASSOC CANCER RESEARCH; PHILADELPHIA
- Number of pages
- 3
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:001373388900095
- Other Identifier
- 991021963115404721
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- Web of Science research areas
- Oncology