Abstract
Arimoclomol® enhances hepatic stress protein accumulation after an acute bout of heat stress
The FASEB journal, v 24(S1), pp 1000.8-1000.8
Apr 2010
Abstract
Abstract only Exposure to extreme environmental conditions can adversely affect homeostasis and lead to serious health consequences. The induction of heat shock proteins (HSPs) in response to stressors maintains cellular and physiological homeostasis. Arimoclomol® (CytRx Corp., Los Angeles, CA) is a co‐inducer of HSP, and while positive effects of Arimoclomol® have been demonstrated in pathological models, less is known about its role in a physiologically relevant stressor such as hyperthermia. The purpose of this study was to evaluate the effects of Arimoclomol® on the accumulation of hepatic HSP after a single bout of heat stress. Male Sprague‐Dawley rats were injected with 200 mg/kg Arimoclomol® (or saline) 1 hour prior to hyperthermia, and at 24‐hour intervals thereafter. HSP expression was assessed via immunoblotting and immunohistochemistry, and we observed a slight increase in HSP27, HSP32 and HSP70 protein levels in the vehicle‐treated groups. Arimoclomol® greatly enhanced accumulation of these HSP, and their levels were maintained for three days after heat stress. This sustainment of HSP expression with the combination of Arimoclomol® and heat stress demonstrates a novel mechanism of preconditioning that may be useful in preventing heat‐related injury. Supported by the Defense Advanced Research Projects Agency (DARPA), contract number W911NF‐07‐C‐0053.
Metrics
14 Record Views
Details
- Title
- Arimoclomol® enhances hepatic stress protein accumulation after an acute bout of heat stress
- Creators
- Steven A Bloomer - University of IowaIbolja Cernak - Johns Hopkins University Applied Physics LaboratoryJodie L Haak - University of IowaKevin C Kregel - University of Iowa
- Publication Details
- The FASEB journal, v 24(S1), pp 1000.8-1000.8
- Publisher
- Wiley
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Health Sciences
- Other Identifier
- 991021888929804721