Abstract
Autoantibody-mediated generation of platelet-bound C4d in Systemic Lupus Erythematosus (SLE)
The Journal of immunology (1950), v 196(1_Supplement), pp 124-124.24
01 May 2016
Abstract
Abstract
Premature and accelerated onset of atherothrombotic disease is prevalent in patients with SLE. Most, if not all, atherothrombotic diseases are likely to involve platelets and the complement system in some capacity. Previously, we discovered that platelets bearing complement activation product C4d (P-C4d) are present in approximately 20% of SLE patients and are significantly associated with anti-phospholipid (aPL) antibody positivity and stroke in SLE patients. The goal of the present study was to elucidate the role of aPL and other platelet-reactive autoantibodies in the generation of P-C4d.
To determine the association between P-C4d and aPL, the level of P-C4d in 180 SLE patients was measured by flow cytometry. Serum level of aPL, including anti-cardiolipin (aCL) and anti-b2 glycoprotein I (anti-b2GPI) antibodies, in these patients was measured by enzyme-linked immunosorbent assays. To investigate the role of aPL, and possibly other autoantibodies as well, in mediating the generation of P-C4d, in vitro 2-step transfer experiments using normal platelets in combination with plasma or immunoglobulin derived from SLE patients were performed. The results showed that the presence and levels of serum aCL and anti- anti-b2GPI were highly specific for SLE patients with positive P-C4d. The plasma and immunoglobulins purified from SLE patients who were positive for P-C4d and aCL/anti-b2GPI were able to induce C4d deposition on normal platelets in vitro. The capacity of SLE plasma in inducing P-C4d appeared to correlate proportionately to the serum aCL levels. Collectively, the results demonstrate that both aPL, particularly aCL, and other platelet-reactive autoantibodies participate in mediating the generation of P-C4d in SLE patients.
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Details
- Title
- Autoantibody-mediated generation of platelet-bound C4d in Systemic Lupus Erythematosus (SLE)
- Creators
- Chau-Ching LiuTravis Schofield - Saint Francis UniversitySusan ManziJoseph M. Ahearn
- Publication Details
- The Journal of immunology (1950), v 196(1_Supplement), pp 124-124.24
- Publisher
- American Association of Immunologists (AAI)
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Medicine (Graduate); General Internal Medicine
- Other Identifier
- 991021933902904721