Abstract
Cell cycle regulator Cyclin D1 is essential for dendritic cell differentiation
European journal of immunology, v 48(Supplement 1), pp 174-174
May 2018
Abstract
Dendritic cells (DCs) are important mediators of the innate and adaptive immune responses. However,the network of genes governing the differentiation of these cells has been a topic of discussion for along time, mainly because of their similarity to other myeloid lineages, such as monocytes and macrophages. DC differentiation was induced ex vivo using a Fms-like tyrosine kinase 3 ligand (Flt3L)dependent culture resulting in the formation of bone marrow-derived CD11c+ DCs (BMDCs). To gain insights into the epigenetic processes and signaling networks that govern DC differentiation we performed DNA methylation and transcriptome profiling on distinct stages of the differentiation process. Distinct CpG dinucleotides demonstrated dynamic methylation changes during differentiation.Transcriptome analysis identified a reduction in gene expression of developmental signaling pathways including the Wnt pathway that included decreased expression of Wnt5a and an increase in the expression of Cyclin D1 (Ccnd1). Differentiation of BMDC in the absence of Ccnd1 resulted in a reduction in CD11c+ cells suggesting a role for Ccnd1 in governing differentiation of DCs.
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Details
- Title
- Cell cycle regulator Cyclin D1 is essential for dendritic cell differentiation
- Creators
- Christopher Scharer (General Contributor) - Emory UniversityRashida Ginwala (General Contributor) - Drexel University, Microbiology and ImmunologyYuka Kawasawa - Penn State Milton S. Hershey Medical CenterKiran Kumar Madugula - Drexel University, Microbiology and ImmunologyDe Gaulle I Chigbu - Drexel University, Microbiology and ImmunologyZafar K Khan - Drexel University, Microbiology and ImmunologyRichard G Pestell - Baruch S. Blumberg InstituteJeremy Boss - Emory UniversityPooja Jain - Drexel University, Microbiology and Immunology
- Publication Details
- European journal of immunology, v 48(Supplement 1), pp 174-174
- Conference
- 15th International Symposium on Dendritic Cells, 15th (Aachen, Germany, 10 Jun 2018–14 Jun 2018)
- Publisher
- Wiley
- Number of pages
- 1
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Microbiology and Immunology; Pennsylvania College of Optometry (PCO)
- Web of Science ID
- WOS:000434106700263
- Scopus ID
- 2-s2.0-85059799188
- Other Identifier
- 991022040867604721
InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Immunology