Abstract
Complement-tagged basophils and eosinophils in Systemic Lupus Erythematosus (SLE)
The Journal of immunology (1950), v 196(1_Supplement), pp 124-124.22
01 May 2016
Abstract
Abstract
Cell-bound complement activation products (CB-CAPs) on erythrocytes, reticulocytes, platelets, and lymphocytes have been recognized as biomarkers for SLE diagnosis, monitoring, and prognosis and to render such cells dysfunctional. Recently, basophils have been shown to contribute to the development of lupus nephritis and presence of eosinophils in the urine has been reported as a potential biomarker for lupus nephritis. This study was based on the hypothesis that basophils and eosinophils bearing C4d may serve as valuable biomarkers and participate in lupus pathogenesis.
To verify this hypothesis, a pilot study investigating basophil-bound and eosinophil-bound C4d levels was conducted using blood samples taken from 103 patients with SLE, 23 patients with other immune-inflammatory diseases, 17 patients with asthma or allergic rhinitis, and 17 healthy individuals. Peripheral blood leukocytes were isolated, and basophils and eosinophils therein were detected and analyzed for surface-bound C4d by flow cytometry.
Preliminary results showed that basophil-bound C4d (Baso-C4d) and eosinophil-bound C4d (Eos-C4d) levels were significantly elevated in patients with SLE, compared to patients with other immune-inflammatory diseases, patients with asthma/allergy, and healthy controls (all p < 0.05). As diagnostic tools, Eos-C4d and Baso-C4d, respectively, were 29% sensitive/96% specific and 22% sensitive/96% specific in differentiating SLE from other immune-inflammatory diseases. The results obtained indicate that Baso-C4d and Eos-C4d may serve as specific markers for SLE diagnosis and possibly for SLE global/organ-specific disease activity.
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Details
- Title
- Complement-tagged basophils and eosinophils in Systemic Lupus Erythematosus (SLE)
- Creators
- Chau-Ching LiuBerryhill E. McCarty - Carnegie Mellon UniversityMeaghan McGeary - College of the Holy CrossSusan ManziJoseph M. Ahearn
- Publication Details
- The Journal of immunology (1950), v 196(1_Supplement), pp 124-124.22
- Publisher
- American Association of Immunologists (AAI)
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Medicine (Graduate); General Internal Medicine
- Other Identifier
- 991021933903104721