Abstract
FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques
European journal of immunology, Vol.48(Supplement 1), pp.175-175
May 2018
Abstract
Cerebrospinal fluid (CSF) drains via the lymphatic drainage pathway. This lymphatic pathwayconnects the central nervous system (CNS) to the cervical lymph node (CLN). As the CSF drains toCLN via the dural and nasal lymphatics, T cells and antigen presenting cells pass along the channelsfrom the subarachnoid space through the cribriform plate. Human immunodeficiency virus (HIV) mayalso egress from the CNS along this pathway. As a result, HIV egressing from the CNS mayaccumulate within the CLN. Towards this objective, we analyzed CLNs isolated from rhesusmacaques that were chronically-infected with simian immunodeficiency virus (SIV). We detectedsignificant accumulation of SIV within the CLNs. SIV virion trapping was observed on folliculardendritic cells (FDCs) localized within the follicular regions of CLNs. In addition, SIV antigens formedimmune complexes when FDCs interacted with B cells within the germinal centers. Subsequentinteraction of these B cells with CD4+ T follicular helper cells (TFHs) resulted in infection of the latter.Of note, 73% to 90% of the TFHs cells within CLNs were positive for SIV p27 antigen. As such, itappears that not only do the FDCs retain SIV they also transmit them (via B cells) to TFHs within theseCLNs. This interaction results in infection of TFHs in the CLNs. Based on these observations, we inferthat FDCs within the CLNs have a novel role in SIV entrapment with implications for viral trafficking.
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Details
- Title
- FDC:TFH Interactions within Cervical Lymph Nodes of SIV-Infected Rhesus Macaques
- Creators
- Rajnish S Dave - Drexel University, Microbiology and ImmunologyRavi K. Sharma - Drexel University, Microbiology and ImmunologyRoshell Muir - Drexel University, Infectious Diseases (and HIV Medicine)Elias El Haddad - Drexel University, Infectious Diseases (and HIV Medicine)Sanjeev Gumber - Emory UniversityFrancois Villinger - University of Louisiana at LafayetteArtinder P Nehra - Drexel University, Microbiology and ImmunologyZafar K Khan - Drexel University, Microbiology and ImmunologyBrian Wigdahl - Drexel University, Microbiology and ImmunologyAftab A Ansari - Emory UniversitySiddappa N Byrareddy - University of Nebraska Medical CenterPooja Jain - Drexel University, Microbiology and Immunology
- Publication Details
- European journal of immunology, Vol.48(Supplement 1), pp.175-175
- Conference
- 15th International Symposium on Dendritic Cells, 15th (Aachen, Germany, 10 Jun 2018–14 Jun 2018)
- Publisher
- Wiley
- Number of pages
- 1
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Microbiology and Immunology; Infectious Diseases (and HIV Medicine)
- Identifiers
- 991022040867404721