Abstract
Fractalkine donwregulates Neural Cell Adhesion Molecule ‐NCAM 180‐ and PSA‐NCAM in cortical neurons 6 DIV: regulation of chemokines upon neuroplastic changes in the rat prefrontal cortex in “in vivo” and “in vitro”
The FASEB journal, v 22(S2), pp 449-449
Apr 2008
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Chemokines are constitutively expressed by neurons and glia in the CNS. SDF‐1, ligand for CXCR4, plays a critical role in neuronal migration and axon pathfinding. On the other hand, NCAM ‐Neural Cell Adhesion Molecule shows a post‐translational modification, PSA‐NCAM, that regulates neural cell‐cell interactions. We studied whether SDF 1 alpha and Fractalkine could regulate NCAM and PSA‐NCAM protein levels in cortical neurons 6 DIV overnight treated with these recombinant chemokines. Our results displayed that fractalkine donwregulated PSA‐NCAM and NCAM 140 protein levels vs their controls. Interestingly, axonal branching and PSA‐NCAM inmunofluorescence decreased after fractalkine treatment. Besides, we evaluated whether chemokines could play a role in learning and memory. SDF1 alpha inmunoreactivity increased in the hippocampus and prefrontal cortex of rats with improved behavioural performance. Interestingly, AMD3100 ‐CXCR4 antagonist‐ infusion before learning, impairs memory consolidation. In conclusion, these findings suggest a role of fractalkine as cell adhesion molecule in the CNS throuough PSA‐NCAM regulation in the rat prefrontal cortex “in vivo” and “in vitro”
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Details
- Title
- Fractalkine donwregulates Neural Cell Adhesion Molecule ‐NCAM 180‐ and PSA‐NCAM in cortical neurons 6 DIV: regulation of chemokines upon neuroplastic changes in the rat prefrontal cortex in “in vivo” and “in vitro”
- Creators
- José Joaquín Merino - Drexel UniversityZaffard Khan - Drexel UniversityFernando Bartolomé - Molecular PhysiopathologyCIB (CSIC)MadridSpainAlexando Fatatis - Drexel UniversityOlimpia Meucci - Drexel University
- Publication Details
- The FASEB journal, v 22(S2), pp 449-449
- Publisher
- Federation of American Societies for Experimental Biology
- Number of pages
- 1
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Microbiology and Immunology; Pharmacology and Physiology
- Web of Science ID
- WOS:000208467700497
- Other Identifier
- 991019168086804721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemistry & Molecular Biology
- Biology
- Cell Biology