Abstract
Protein kinase C isoforms α, βII and δ are expressed in gallbladder epithelium during gallstone formation
Gastroenterology (New York, N.Y. 1943), v 120(5 supplement 1), pp A386-A386
Apr 2001
Abstract
Background and Aims: Gallbladder(GB) Na" absorption and biliary Ca z+ are both increased duriog gallstone(GS) formation. Luminal Ca ~- regulates GB ion transport via intracellular Ca 2. [Ca2* ], in prairie dogs. Recent data suggest that Ca z÷ homeostasis in GB epithelium is altered in early GS formation and may result in increased [CaZ*], (submitted to DDW 2001). [Ca2+]~ mediates its effects through second messenger systems including protein kinase C (PKC). At least 12 PKC isoforms have been identified, broadly divided into three main groups based on their relative requirements for [Ca2*],, diacylgylceride and phospholipid for activation. The classical group, including PKC~z and/3, requires all three factors, while the novel group, which includes PKCS, is CaZ+-independant, and the atypical group requires phospholipid only. Expression of these isoforms is species and tissue specific, though, to date, no specific PKC isoforms have been identified in GB. The aim of the present study was to examine whether Ca 2--dependent PKC isoforms are expressed by GB epithelium. Methods: GBs were harvested from both patients undergoing cholecystectomy and prairie dogs fed nonlithogenic diet. Total RNA was extracted from GB epithelia, reverse transcribed, and amplified by PCR using PKC isoform-specific primers. PCR products were cloned using the TA cloning kit (Invitrogen), and sequence analysis was performed on cDNA fragments. Results: RT-PCR analysis shows the GB expression of PKC(x,/~11 and 8 mRNA in human (Fig) and prairie dog (not shown) gallbladders. Nucleotide sequence analysis of prairie dog PKCtz (443 bp),/311 (309 bp) and 8 (260 bp) demonstrated a -90% homology to human, rat and rabbit. The deduced amino acid sequences were also highly identical to human, rat and rabbit. Conclusions: This is the first report that human GB expresses both Ca 2 *-dependent PKCo~ and/~ll and Ca 2. -Independent PKC8 isoforms during GS formation. Further studies are warranted to investigate the relative expression of these isoforms and their regulation of Na + transport during gallstone formation.
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Details
- Title
- Protein kinase C isoforms α, βII and δ are expressed in gallbladder epithelium during gallstone formation
- Creators
- Seth C Narins - Drexel University, Medical College of Pennsylvania and Hahnemann University (1993-1996, 1998-2002)Eun H Park - Drexel University, Medical College of Pennsylvania and Hahnemann University (1993-1996, 1998-2002)Xuefeng Su - University of Alabama at BirminghamPeter R Smith - University of Alabama at BirminghamMohammad Z Abedin - Drexel University, Medical College of Pennsylvania and Hahnemann University (1993-1996, 1998-2002)
- Publication Details
- Gastroenterology (New York, N.Y. 1943), v 120(5 supplement 1), pp A386-A386
- Conference
- Digestive Disease Week and the 102nd Annual Meeting of the American Gastroenterological Association, 102nd (Atlanta, Georgia, United States, 20 May 2001–23 May 2001)
- Publisher
- Elsevier
- Number of pages
- 1
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- College of Medicine
- Other Identifier
- 991019318925304721