Abstract
The utilization of humanized mouse models for the study of human retroviral infections
Retrovirology, v 6(1), pp 76-76
12 Aug 2009
PMID: 19674458
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The development of novel techniques and systems to study human infectious diseases in both an in vitro and in vivo settings is always in high demand. Ideally, small animal models are the most efficient method of studying human afflictions. This is especially evident in the study of the human retroviruses, HIV-1 and HTLV-1, in that current simian animal models, though robust, are often expensive and difficult to maintain. Over the past two decades, the construction of humanized animal models through the transplantation and engraftment of human tissues or progenitor cells into immunocompromised mouse strains has allowed for the development of a reconstituted human tissue scaffold in a small animal system. The utilization of small animal models for retroviral studies required expansion of the early CB-17 scid/scid mouse resulting in animals demonstrating improved engraftment efficiency and infectivity. The implantation of uneducated human immune cells and associated tissue provided the basis for the SCID-hu Thy/Liv and hu-PBL-SCID models. Engraftment efficiency of these tissues was further improved through the integration of the non-obese diabetic (NOD) mutation leading to the creation of NODSCID, NOD/Shi-scid IL2r gamma(-/-),and NOD/SCID beta 2-microglobulin(null) animals. Further efforts at minimizing the response of the innate murine immune system produced the Rag2(-/-)gamma(-/-)(c) model which marked an important advancement in the use of human CD34+ hematopoietic stem cells. Together, these animal models have revolutionized the investigation of retroviral infections in vivo.
Metrics
Details
- Title
- The utilization of humanized mouse models for the study of human retroviral infections
- Creators
- Rachel Van Duyne - George Washington UniversityCaitlin Pedati - George Washington UniversityIrene Guendel - George Washington UniversityLawrence Carpio - George Washington UniversityKylene Kehn-Hall - George Washington UniversityMohammed Saifuddin - Eastern Virginia Medical SchoolFatah Kashanchi - George Washington University
- Publication Details
- Retrovirology, v 6(1), pp 76-76
- Publisher
- Springer Nature
- Number of pages
- 18
- Grant note
- HRN-A-00-98-00020-00 / Eastern Virginia Medical School under a Cooperative Agreement MSA-06-437 / CONRAD; United States Agency for International Development (USAID) United States Agency for International Development ( USAID); United States Agency for International Development (USAID); CGIAR R21AI071903 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) AI071903-01 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Abstract
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000270145500001
- Scopus ID
- 2-s2.0-70349918640
- Other Identifier
- 991021902526204721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Virology