Book chapter
[15] Modulated excitation spectroscopy in hemoglobin
Methods in Enzymology, pp.292-321
Elsevier Science & Technology
1994
PMID: 8057866
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Abstract
This chapter focuses on the modulated excitement spectroscopy in hemoglobin (Hb). Modulated excitation is a kinetic perturbation method that uses frequency-domain techniques to observe small relaxations that may be partially masked by larger amplitude processes of lesser interest. Because it is basically a repetitive technique, it has found use thus far in reversible photolysis reactions, and there it has been used to study conformational change in Hb. This chapter also discusses the pulse methods to set the scope of the kinetic problems that are addressed by modulated excitation. Modulated excitation uses a beam that is repeatedly turned on and off, with observation of the accompanying optical changes. In this sense it is a means of performing the necessary averaging. Stability is assured by modulating a constant light output, rather than requiring pulse-to-pulse repetition. However, an unexpected bonus appears in the ability to adjust the phase of the detection system.
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Details
- Title
- [15] Modulated excitation spectroscopy in hemoglobin
- Creators
- Frank A Ferrone
- Publication Details
- Methods in Enzymology, pp.292-321
- Publisher
- Elsevier Science & Technology
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Physics
- Identifiers
- 991014878428404721
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- Biochemical Research Methods
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