Book chapter
[23] Ligand-binding assays in Xenopus oocytes
Methods in Enzymology, pp 368-375
1992
PMID: 1382192
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Abstract
This chapter discusses the applications of ligand-binding assays to ion channels and receptors expressed in Xenopus oocytes and presents some general considerations for ligand-binding assays using oocytes. Ligand-binding assays are performed by incubating intact cells, cell extracts, or purified membranes with a radiolabeled ligand for a period of time that is sufficient for equilibrium to be attained. The receptor–ligand complex is separated from the unbound ligand and the amount bound is quantified by liquid scintillation or γ counting. The chapter describes two different protocols for binding assays; cell surface receptor binding of [125I]SARILE to angiotensin II receptors and 125I-labeled α-Bungarotoxin binding to acetylcholine receptors in oocyte extracts, which is used to determine the total number of acetylcholine receptors expressed in oocytes after the injection of in vitro transcripts.
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Details
- Title
- [23] Ligand-binding assays in Xenopus oocytes
- Creators
- Amy L. BullerMichael M. White
- Publication Details
- Methods in Enzymology, pp 368-375
- Publisher
- Elsevier Science & Technology
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1992JM50000023
- Scopus ID
- 2-s2.0-0026644284
- Other Identifier
- 991020837831904721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology