Book chapter
Association of bacteria with human phagocytes
Methods in Enzymology, v 253, pp 12-26
1995
PMID: 7476381
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This chapter presents methods and approaches to study and quantify the adhesion of bacteria to human phagocytic cells, specifically polymorphonuclear neutrophils (PMNs), monocytes, and macrophages. Adherent bacteria are often, but not always, internalized by the phagocytes to which they adhere. The assays presented in the chapter consist of incubating bacteria and phagocytes, sometimes with serum or other body fluids, and then quantifying adhesion. Bacteria attach to phagocytes by two broad mechanisms: (1) directly, by using specific molecules expressed on outer cell surfaces such as fimbriae (pili) and nonfimbrial ligands, often called “adhesins,” such as the Opa proteins of Neisseria gonorrhoeae, and (2) indirectly, by binding host components, most often immunoglobulins and complement components, which subsequently bind to phagocyte receptors. Regardless of the mechanism, the methods used to quantify adhesion are similar. The ability of bacteria to bind to phagocytes can vary tremendously depending on the bacterial strain or variant—and growth conditions and growth phase—among other variables. Many bacterial surface ligands vary independently of growth conditions or phase; such variations are not rare events.
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Details
- Title
- Association of bacteria with human phagocytes
- Creators
- Richard F Rest - Drexel University
- Publication Details
- Methods in Enzymology, v 253, pp 12-26
- Publisher
- Elsevier Science & Technology
- Number of pages
- 15
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:A1995BE08D00002
- Scopus ID
- 2-s2.0-0029035627
- Other Identifier
- 991019184048504721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Cell Biology