Book chapter
Autoradiographic localization of newly synthesized inositol phospholipids in the superior cervical ganglion
Methods in Enzymology
1987
PMID: 3600357
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Muscarine stimulates the hydrolysis of inositol-containing phospholipids and increases the activity of tyrosine hydroxylase in the ganglion. Vasopressin also stimulates the hydrolysis of inositol-containing phospholipids in the ganglion but does not increase the activity of tyrosine hydroxylase (tyrosine 3-monooxygenase) in this tissue. Most of the tyrosine hydroxylase in the ganglion is localized in the cell bodies of the principal ganglionic neurons. Establishing a role for phospholipid turnover in the mechanism by which muscarine increases tyrosine hydroxylase activity requires a demonstration that muscarine increases phospholipid metabolism in these cell bodies and that vasopressin increases phospholipid metabolism in some other compartment within the ganglion. The chapter explains the isolation and incubation of ganglia. To study the effects of agonists on inositol phospholipid synthesis, the ganglia are incubated in Earle's Balanced Salt Solution (EBSS)-containing [3H]inositol, in the presence or absence of the desired agonist. When ganglia are incubated with [3H]inositol in the presence of low concentrations of carrier inositol, most of the radioactivity is taken up into cells near the surface of the ganglion. The chapter describes autoradiography. It is a procedure that requires a darkroom, histological processing equipment, and a separate refrigerator to store the autoradiograms during exposure.
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Details
- Title
- Autoradiographic localization of newly synthesized inositol phospholipids in the superior cervical ganglion
- Creators
- Conwell H. AndersonJoel HorwitzRobert L. Perlman
- Publication Details
- Methods in Enzymology
- Publisher
- Elsevier Science & Technology
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1987J147900018
- Scopus ID
- 2-s2.0-0023079393
- Other Identifier
- 991020531826004721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology