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Book chapter
Branch Migration Activity of Rad54 Protein: Methods and Protocols
01 Jan 2021
PMID: 32840778
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Rad54 is a eukaryotic protein that plays an important role in homologous recombination. Rad54, a member of the Swi2/Snf2 family, binds to Holliday junctions with high specificity and promotes their branch migration in an ATP hydrolysis-dependent manner. Here we describe the methods our laboratory used to characterize the branch migration activity of Rad54. These assays are applicable for other branch migration proteins regardless of whether they have canonical helicase activity or not.
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Details
- Title
- Branch Migration Activity of Rad54 Protein
- Creators
- Olga M. Mazina - Drexel UniversityAlexander V. Mazin - Drexel University
- Contributors
- A Aguilera (Editor)A Carreira (Editor)
- Series
- Methods in Molecular Biology
- Publisher
- Humana Press Inc; TOTOWA
- Number of pages
- 23
- Grant note
- Drexel-Coulter Program Award CA188347; P30CA056036 / National Cancer Institute of the National Institutes of Health (NIH); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000679479500012
- Scopus ID
- 2-s2.0-85089923293
- Other Identifier
- 991019168376904721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology