Book chapter
DNA Sequence Amplification in Mammalian Cells
International Review of Cytology, pp 31-82
1984
PMID: 6389416
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This chapter describes DNA sequence amplification in mammalian cells. The synthesis of a chromosomal DNA fiber occurs through the agency of thousands of tandemly arranged replicons, each of which usually functions only once in a given S period to ensure that the fiber is exactly duplicated along its entire length. The result of this process is that the two identical daughter chromatids lay side-by-side, connected by a centromere, until the separation of chromatids occurs at mitosis and the ploidy of each daughter cell is restored to the original configuration. There are exceptions to this mode of replication, however, in which, parts of chromosomes or the entire chromosomal complement are rereplicated prior to a cell division event, with the consequence that the genetic constitution of the cell can be increased in total, or only at selected loci. This chapter illustrates examples of selective DNA amplification in both prokaryotic and eukaryotic systems. It discusses the cytological manifestations of gene amplification and the stability of the process.
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Details
- Title
- DNA Sequence Amplification in Mammalian Cells
- Creators
- Joyce L. Hamlin - University of VirginiaJeffrey D. Milbrandt - University of VirginiaNicholas H. Heintz - University of VirginiaJane C. Azizkhan - University of Virginia
- Publication Details
- International Review of Cytology, pp 31-82
- Publisher
- Elsevier Science & Technology
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:A1984TQ99600002
- Scopus ID
- 2-s2.0-0021646562
- Other Identifier
- 991020200887904721
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Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell Biology