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Book chapter
Detecting Waterborne Parasites Using Piezoelectric Cantilever Biosensors
Biosensors and Environmental Health, pp 318-335
01 Jan 2012
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Molecular and immuno-based methods for detecting waterborne parasites in current use are both time-consuming and laborious. A great need exists for developing rapid and inexpensive methods for water quality and environmental monitoring. In this chapter, we review the application of piezoelectric-excited millimeter-sized cantilever (PEMC) sensor for the detection of waterborne parasites. Cantilever physics and working principle, sensor fabrication and surface functionalization, flow experimental design, as well as examples of detecting C. parvum and G. lamblia are presented. PEMC sensor is a mass-sensitive biosensor whose resonant frequency decreases when the mass of the sensor increases due to target binding. PEMC sensor functionalized with a specific antibody was exposed to target parasites in a flow apparatus. When the parasite binds to the surface-immobilized antibody, mass of the sensor increases and causes decrease of sensor resonant frequency. Real-time monitoring of resonant frequency changes was used for low Limit of detection (LOD) for C. parvum using PEMC sensor was 5 oocysts/mL in 25% milk medium. In the dynamic range of 50 to 10,000 oocysts/mL the sensor response is characterized by a semi-log relationship between resonant frequency response and C. parvum oocysts concentration. In 25% milk background, both binding kinetics was slower and total sensor response was lower (similar to 45%) than in water-like medium. LOD for G. lamblia was 10 cysts/mL in both buffer and complex matrixes (tap water and river water). Feasibility of analyzing at a low concentration of 1 cyst/mL in a one liter sample is also described.
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Details
- Title
- Detecting Waterborne Parasites Using Piezoelectric Cantilever Biosensors
- Creators
- Sen Xu - Drexel Univ, Dept Chem & Biol Engn, Philadelphia, PA 19104 USARaj Mutharasan - Drexel Univ, Dept Chem & Biol Engn, Philadelphia, PA 19104 USA
- Contributors
- V R Preedy (Editor)V B Patel (Editor)
- Publication Details
- Biosensors and Environmental Health, pp 318-335
- Publisher
- Taylor & Francis; BOCA RATON
- Number of pages
- 18
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Chemical and Biological Engineering
- Web of Science ID
- WOS:000387294600018
- Other Identifier
- 991020950672904721
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- Web of Science research areas
- Chemistry, Analytical
- Environmental Sciences