Book chapter
Image Contrast in Confocal Light Microscopy
Handbook of Biological Confocal Microscopy
1995
Abstract
In any form of microscopy, one needs not only an imaging system with enough resolution to reveal the fine details of a specimen but also a suitable contrast mechanism by which to “see” the structures of interest. As defined by the New Webster ′s Dictionary, contrast is the difference between light and dark areas of a negative or print. In other words, contrast is the difference in signal strength between various parts of an image or between details of interest and “background.” In science, the contrast is proportional to the intensity difference (ΔI) between two image areas, divided by the average image brightness Ī.
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Details
- Title
- Image Contrast in Confocal Light Microscopy
- Creators
- P. C. Cheng - University at Buffalo, State University of New YorkA. Kriete - Justus-Liebig-Universität Gießen
- Publication Details
- Handbook of Biological Confocal Microscopy
- Publisher
- Springer US; Boston, MA
- Number of pages
- 30
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Other Identifier
- 991019778119104721