Book chapter
Measurement of inositol phospholipid metabolism in PC12 pheochromocytoma cells
Methods in Enzymology
1987
PMID: 3298960
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Abstract
PC12 cells are a clonal cell line derived from a rat pheochromocytoma. These cells are widely used as models for both adrenal chromaffin cells and sympathetic neurons. When PC 12 cells are grown in the absence of nerve growth factor (NGF), they appear as small, round, undifferentiated cells. In the presence of NGF, the cells stop dividing, enlarge, and extend long neuronal-like processes. NGF also increases the number of muscarinic cholinergic receptors on PC12 cells. Muscarinic agonists stimulate the metabolism of inositol-containing phospholipids in PC12 cells. The ability to grow large amounts of a homogeneous population of cells, to manipulate the properties of the cells by changing the extracellular environment, and to stimulate phospholipid metabolism with specific agonists provides valuable opportunities for studying neuronal phospholipid metabolism in PC12 cells. The chapter describes the growth PC12 cells.
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Details
- Title
- Measurement of inositol phospholipid metabolism in PC12 pheochromocytoma cells
- Creators
- Joel HorwitzRobert L. Perlman
- Publication Details
- Methods in Enzymology
- Publisher
- Elsevier Science & Technology
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:A1987J147900015
- Scopus ID
- 2-s2.0-0023161275
- Other Identifier
- 991020532110104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology