Book chapter
Microfluidic "Pouch" Chips for Immunoassays and Nucleic Acid Amplification Tests
Biosensors and Biodetection, pp 467-488
01 Jan 2017
PMID: 28299706
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Microfluidic cassettes ("chips") for processing and analysis of clinical specimens and other sample types facilitate point-of-care (POC) immunoassays and nucleic acid based amplification tests. These single-use test chips can be self-contained and made amenable to autonomous operation-reducing or eliminating supporting instrumentation-by incorporating laminated, pliable "pouch" and membrane structures for fluid storage, pumping, mixing, and flow control. Materials and methods for integrating flexible pouch compartments and diaphragm valves into hard plastic (e.g., acrylic and polycarbonate) microfluidic "chips" for reagent storage, fluid actuation, and flow control are described. We review several versions of these pouch chips for immunoassay and nucleic acid amplification tests, and describe related fabrication techniques. These protocols thus offer a "toolbox" of methods for storage, pumping, and flow control functions in microfluidic devices.
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Details
- Title
- Microfluidic "Pouch" Chips for Immunoassays and Nucleic Acid Amplification Tests
- Creators
- Michael G. Mauk - Univ Penn, Sch Engn & Appl Sci, Philadelphia, PA 19104 USAChangchun Liu - University of PennsylvaniaXianbo Qiu - Beijing University of Chemical TechnologyDafeng Chen - University of PennsylvaniaJinzhao Song - University of PennsylvaniaHaim H. Bau - University of Pennsylvania
- Contributors
- B Prickril (Editor)A Rasooly (Editor)
- Publication Details
- Biosensors and Biodetection, pp 467-488
- Series
- Methods in Molecular Biology
- Publisher
- Springer Nature; DORDRECHT
- Number of pages
- 22
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Engineering Technology
- Web of Science ID
- WOS:000444594800031
- Scopus ID
- 2-s2.0-85015636922
- Other Identifier
- 991020623909604721
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- Collaboration types
- Domestic collaboration
- International collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology