Book chapter
Microprinting of Liver Micro-organ for Drug Metabolism Study
Biological Microarrays, pp 219-238
01 Jan 2011
PMID: 20967633
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In their normal in vivo matrix milieu, tissues assume complex well-organized 3D architectures. Therefore, a primary aim in the tissue engineering design process is to fabricate an optimal analog of the in vivo scenario, in which the precise configuration and composition of cells and bioactive matrix components can establish the well-defined biomimetic microenvironments that promote cell cell and cell matrix interactions. With the advent and refinements in microfabricated systems which can present physical and chemical cues to cells in a controllable and reproducible Fashion unrealizable with conventional tissue culture, high-fidelity, high-throughput in vitro models are achieved. The convergence of solid freeform fabrication (SFF) technologies, namely microprinting, along with microfabrication techniques, a 3D microprinted Micro-organ, can serve as an in vitro platform for cell culture, drug screening, or to elicit further biological insights. This chapter firstly details the principles, methods, and applications that undergird the fabrication process development and adaptation of microfluidic devices for the creation of a drug screening model. This model involves the combinatorial setup of an automated syringe-based, layered direct cell writing microprinting process with soft lithographic micropatterning techniques to fabricate a microscale in vitro device housing a chamber of microprinted 3D micro-organ that biomimics the cell's natural microenvironment for enhanced performance and functionality. In order to assess the structural formability and biological feasibility of such a micro-organ, 3D cell-encapsulated hydrogel-based tissue constructs are microprinted reproducibly in defined design patterns and biologically characterized for both viability and cell-specific function. Another key fleet of the in vivo microenvironment that is recapitulated with the in vitro system is the necessary dynamic perfusion of the 3D microscale liver analog with cells probed for their collective drug metabolic function and suitability as a drug metabolism model.
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Details
- Title
- Microprinting of Liver Micro-organ for Drug Metabolism Study
- Creators
- Robert C. Chang - Drexel UniversityKamal Emami - Johnson Space CenterAntony Jeevarajan - Johnson Space CenterHonglu Wu - Johnson Space CenterWei Sun - Drexel University
- Contributors
- A Khademhosseini (Editor)K Y Suh (Editor)M Zourob (Editor)
- Publication Details
- Biological Microarrays, pp 219-238
- Series
- Methods in Molecular Biology
- Publisher
- Humana Press Inc; TOTOWA
- Number of pages
- 20
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Web of Science ID
- WOS:000284031200013
- Scopus ID
- 2-s2.0-79952200356
- Other Identifier
- 991019167572404721
UN Sustainable Development Goals (SDGs)
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology