Book chapter
Overview of Glutamine Dependency and Metabolic Rescue Protocols: Methods and Protocols
pp 427-439
01 Jan 2019
PMID: 30725468
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Enhanced glutaminolysis and glycolysis are the two most remarkable biochemical features of cancer cell metabolism, reflecting increased utilization of glutamine and glucose in proliferating cells. Most solid tumors often outgrow the blood supply, resulting in a tumor microenvironment characterized by the depletion of glutamine, glucose, and oxygen. Whereas mechanisms by which cancer cells sense and metabolically adapt to hypoxia have been well characterized with a variety of cancer types, mechanisms by which different types of tumor cells respond to a dynamic change of glutamine availability and the underlying importance remains to be characterized. Here we describe the protocol, which uses cultured Hep3B cells as a model in determining glutamine-dependent proliferation, metabolite rescuing, and cellular responses to glutamine depletion. These protocols may be modified to study the metabolic roles of glutamine in other types of tumor or non-tumor cells as well.
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Details
- Title
- Overview of Glutamine Dependency and Metabolic Rescue Protocols
- Creators
- Shuo Qie - Medical University of South CarolinaDan He - Department of Biology, College of Arts & Sciences, Drexel University, Philadelphia, PA, USA.Nianli Sang - Drexel University
- Contributors
- M Haznadar (Editor)
- Publication Details
- pp 427-439
- Series
- Methods in Molecular Biology
- Publisher
- Humana Press Inc; TOTOWA
- Number of pages
- 13
- Grant note
- R01-CA129494 / NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 81470134 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC) Drexel University
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Biology
- Web of Science ID
- WOS:000683461000023
- Scopus ID
- 2-s2.0-85061122810
- Other Identifier
- 991019167907204721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Oncology