Book chapter
Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters
Functional Genomics, pp 347-359
04 Nov 2011
PMID: 22131004
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In many RNA silencing applications, there is a benefit to expressing multiple interfering RNAs simultaneously. This can be achieved by using a single RNA polymerase II promoter to express multiple micro(mi)RNA-formatted interfering RNAs that are arranged in a polycistronic cluster, mimicking the organization of naturally clustered, endogenous miRNAs. While RNA pol III promoters are often used to express individual short hairpin (sh) RNAs, we have recently shown that pol III promoters can also be used to drive polycistronic expression of miRNA-formatted interfering RNAs. Here, we present methods for the assembly of polycistronic miRNA expression vectors that use pol III promoters. In addition, we present methods for testing the potency and the level of expression of each of the individual miRNAs encoded in the construct.
Metrics
Details
- Title
- Polycistronic Expression of Interfering RNAs from RNA Polymerase III Promoters
- Creators
- Laura F Steel - Department of Microbiology and Immunology, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, USAViraj R Sanghvi - Department of Microbiology and Immunology, Institute for Molecular Medicine and Infectious Disease, Drexel University College of Medicine, Philadelphia, USA
- Publication Details
- Functional Genomics, pp 347-359
- Series
- Methods in Molecular Biology
- Publisher
- Springer New York; New York, NY
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000299396500026
- Scopus ID
- 2-s2.0-84555187853
- Other Identifier
- 991014877924104721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology