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Book chapter
Profiling Axonal mRNA Transport
RNA Detection and Visualization, pp 335-352
2011
PMID: 21431751
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The importance of mRNA localization and localized protein synthesis to spatially modulate protein levels in distinct subcellular domains has increasingly been recognized in recent years. Axonal and dendritic processes of neurons represent separate functional domains of the cell that have shown the capacity to autonomously respond to extracellular stimuli through localized protein synthesis. With the vast distance often separating distal axons and dendrites from the neuronal cell body, these processes have provided an appealing and useful model system to study the mechanisms that drive mRNA localization and regulate localized mRNA translation. Here, we discuss the methodologies that have been used to isolate neuronal processes to purity, and provide an in-depth method for using a modified Boyden chamber to isolate axons from adult dorsal root ganglion neurons for analyses of axonal mRNA content. We further show how this method can be utilized to identify specific mRNAs whose transport into axons is altered in response to extracellular stimuli, providing a means to begin to understand how axonal protein synthesis contributes to the proper function of the neuron.
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Details
- Title
- Profiling Axonal mRNA Transport
- Creators
- Dianna E. Willis - Burke Medical Research InstituteJeffery L. Twiss - Drexel University
- Publication Details
- RNA Detection and Visualization, pp 335-352
- Series
- Methods in Molecular Biology; 714
- Publisher
- Humana Press; Totowa, NJ
- Number of pages
- 18
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000289740700021
- Scopus ID
- 2-s2.0-79960034967
- Other Identifier
- 1617790052; 9781617790058; 991021892106704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Cell Biology