Book chapter
Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone
Human Retroviruses, pp 285-296
01 Jan 2014
PMID: 24158831
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Quantitative PCR (qPCR) provides a robust method for quantifying DNA species. By combining modern qPCR techniques with the isolation of small RNA, the polyadenylation of the RNA, and the use of reverse transcriptase to create miRNA derived cDNA, it is now possible to use qPCR to quantify miRNA. This method is scalable and provides a useful addition to the retrovirologists' toolbox. Here, we also describe the use of one-LTR infectious molecular clones to verify miRNA target sites within the retroviral LTR.
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Details
- Title
- Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone
- Creators
- Zachary A. Klase - National Institute of Allergy and Infectious DiseasesLaurent Houzet - National Institute of Allergy and Infectious DiseasesKuan-Teh Jeang - NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA
- Contributors
- E Vicenzi (Editor)G Poli (Editor)
- Publication Details
- Human Retroviruses, pp 285-296
- Series
- Methods in Molecular Biology
- Publisher
- Humana Press Inc; TOTOWA
- Number of pages
- 12
- Grant note
- Intramural NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Pharmacology and Physiology
- Web of Science ID
- WOS:000326509000025
- Scopus ID
- 2-s2.0-84934443683
- Other Identifier
- 991021902597604721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Immunology
- Virology