Book chapter
Reconstituting the Key Steps of the DNA Double-Strand Break Repair In Vitro
DNA Recombination, pp 407-420
28 Apr 2011
PMID: 21660707
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Double-stranded DNA breaks (DSB), the most harmful type of DNA lesions, cause cell death and genome instability. Homologous recombination repairs DSB using homologous DNA sequences as templates. Here we describe a set of reactions that lead to reconstitution of the double-stranded DNA break repair process in vitro employing purified human homologous recombination proteins and DNA polymerase η. Reconstitution of critical steps of DSB repair in vitro may help to better understand the mechanisms of recombinational DNA repair and the role of various human homologous recombination proteins in this process.
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Details
- Title
- Reconstituting the Key Steps of the DNA Double-Strand Break Repair In Vitro
- Creators
- Matthew J Rossi - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, USADmitry V Bugreev - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, USAOlga M Mazina - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, USAAlexander V Mazin - Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, USA
- Publication Details
- DNA Recombination, pp 407-420
- Series
- Methods in Molecular Biology
- Publisher
- Humana Press; Totowa, NJ
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000292530400023
- Scopus ID
- 2-s2.0-80053330754
- Other Identifier
- 991014878215104721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology