Book chapter
X-Ray Crystallographic Study of the Structure of Prostaglandin H Synthase
Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury 2, pp 107-111
1997
PMID: 9547544
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Prostaglandin H synthase (PGHS) is the first enzyme in the prostaglandin pathway of the arachidonate cascade (1). It is a bifunctional enzyme that catalyzes the conversion of arachidonic acid into prostaglandin H2 (PGH2). Two isozymes of the enzyme (PGHS-1 and PGHS-2) exist and have different modes of expression and tissue distributions (2). The first reaction catalyzed by PGHS, the cyclooxygenase step (3), inserts two molecules of oxygen into arachidonic acid to produce prostaglandin G2 (PGG2). In the second step, the peroxidase reaction reduces the hydroperoxide PGG2 to PGH2. PGHS, a high-spin ferric heme protein with molecular weight of 70 kDa per subunit (3), can form a dimer in solution, but its physiological aggregation state is uncertain. While PGHS is an integral membrane protein, found mainly in the endoplasmic reticulum, its mode of insertion into biological membranes was unknown until recently. In this communication, we described the structure of ovine PGHS-1, recently determined by X-ray crystallography at 3.5 Å (4) using crystals grown in the presence of detergents (5).
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Details
- Title
- X-Ray Crystallographic Study of the Structure of Prostaglandin H Synthase
- Creators
- Daniel Picot - University of ChicagoPatrick J. Loll - University of ChicagoR. Michael Garavito
- Publication Details
- Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury 2, pp 107-111
- Series
- Advances in Experimental Medicine and Biology
- Publisher
- Springer US; Boston, MA
- Resource Type
- Book chapter
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology
- Web of Science ID
- WOS:000072752200015
- Scopus ID
- 2-s2.0-0031430230
- Other Identifier
- 991019295293704721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Medicine, Research & Experimental
- Oncology
- Radiology, Nuclear Medicine & Medical Imaging