Review
Chemogenetic Signaling in Space and Time: Considerations for Designing Neuroscience Experiments Using DREADDs
The Neuroscientist
19 Nov 2022
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The use of designer receptors exclusively activated by designer drugs (DREADDs) has led to significant advances in our understanding of the neural circuits that govern behavior. By allowing selective control over cellular activity and signaling, DREADDs have become an integral tool for defining the pathways and cellular phenotypes that regulate sleep, pain, motor activity, goal-directed behaviors, and a variety of other processes. In this review, we provide a brief overview of DREADDs and discuss notable discoveries in the neurosciences with an emphasis on circuit mechanisms. We then highlight methodological approaches to achieve pathway specific activation of DREADDs. Finally, we discuss spatial and temporal constraints of DREADDs signaling and how these features can be incorporated into experimental designs to precisely dissect circuits of interest.
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Details
- Title
- Chemogenetic Signaling in Space and Time: Considerations for Designing Neuroscience Experiments Using DREADDs
- Creators
- Philip J. Clark - Drexel UniversityZachary D. Brodnik - Drexel UniversityRodrigo A. España - Drexel University
- Publication Details
- The Neuroscientist
- Publisher
- SAGE Publications; Los Angeles, CA
- Grant note
- DA031900-09 / National Institute on Drug Abuse (https://doi.org/10.13039/100000026)
- Resource Type
- Review
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Web of Science ID
- WOS:000889775200001
- Scopus ID
- 2-s2.0-85142239424
- Other Identifier
- 991019306092204721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Clinical Neurology
- Neurosciences