Review
Current trends in molecular recognition and bioseparation
Journal of Chromatography A, v 707(1), pp 3-22
1995
PMID: 7633593
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Molecular recognition guides the selective interaction of macromolecules with each other in essentially all biological processes. Perhaps the most impactful use of biomolecular recognition in separation science has been in affinity chromatography. The results of the last 26 years, since Cuatrecases, Wilchek and Anfinsen first reported the purification of staphylococcal nuclease, have validated the power of biomolecular specificity for purification. This power has stimulated an explosion of solid-phase ligand designs and affinity chromatographic applications. An ongoing case in point is the purification of recombinant proteins, which has been aided by engineering the proteins to contain Affinity-Tag sequences, such as hexa-histidine for metal-chelate separation and epitope sequence for separation by an immobilized monoclonal antibody. Tag technology can be adapted for plate assays and other solid-phase techniques. The advance of affinity chromatography also has stimulated immobilized ligand-based methods to characterize macromolecular recognition, including both chromatographic and optical biosensor methods. And, new methods such as phage display and other diversity library approaches continue to emerge to identify new recognition molecules of potential use as affinity ligands. Overall, it is tantalizing to envision a continued evolution of new affinity technologies which use the selectivity built into biomolecular recognition as a vehicle for purification, analysis, screening and other applications in separation sciences.
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Details
- Title
- Current trends in molecular recognition and bioseparation
- Creators
- Chris Jones - SmithKline BeechamArun Patel - Molecular Discovery (United Kingdom)Skip Griffin - Molecular Discovery (United Kingdom)John Martin - Molecular Discovery (United Kingdom)Peter Young - New FrontierKevin O'Donnell - Molecular Discovery (United Kingdom)Carol Silverman - Molecular Discovery (United Kingdom)Terry Porter - Molecular Discovery (United Kingdom)Irwin Chaiken - New Frontier
- Publication Details
- Journal of Chromatography A, v 707(1), pp 3-22
- Publisher
- Elsevier
- Resource Type
- Review
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; Drexel University
- Web of Science ID
- WOS:A1995RL34100002
- Scopus ID
- 2-s2.0-0029024514
- Other Identifier
- 991019520541904721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical