Conference proceeding
3-D visualization of mammalian intranuclear chromatin using computer-assisted confocal laser scan microscopy
Proceedings of SPIE, v 1357(1), pp 14-18
01 Nov 1990
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
In this study we describe three different approaches for the 3-D reconstruction of the spatial arrangement of iniranuclear chromatin. Using a Zeiss confocal laser scanning microscope (CLSM) image acquisition of optical sections was achieved in the fluorescence mode either using isolated nuclei stained with ethidiumbromide (EthBr) or on Feulgen stained tissue whole mounts. Different methods were applied for 3-D reconstruction: (i) contours of interesting structures were outlined by interactive cursor movement on a digitizer tablet (ii) digitized optical sections were transformed into image stacks by a software implemented on the microscope system and finally processed for a 3-D display and (iii) a ray-tracing method was used to provide a 3-D display of reconstructed surfaces from serial CLSM images after extensive image preprocessing. The characteristics of the different methods are discussed with respect to the biological system used.
Metrics
3 Record Views
Details
- Title
- 3-D visualization of mammalian intranuclear chromatin using computer-assisted confocal laser scan microscopy
- Creators
- M Montag - German Cancer Research CenterHerbert Spring - German Cancer Research CenterMichael F Trendelenburg - German Cancer Research CenterAndres Kriete - Justus-Liebig-Universität Gießen
- Publication Details
- Proceedings of SPIE, v 1357(1), pp 14-18
- Conference
- Medtech '89: Medical Imaging (Campinas, Brazil, 11 Jul 1990–11 Jul 1990)
- Publisher
- Society of Photo-Optical Instrumentation Engineers (SPIE)
- Number of pages
- 5
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:A1990BS33C00002
- Other Identifier
- 991019186661504721
UN Sustainable Development Goals (SDGs)
This publication has contributed to the advancement of the following goals:
InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Medical Laboratory Technology
- Radiology, Nuclear Medicine & Medical Imaging