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3D imaging of lung tissue by confocal microscopy and micro-CT
Conference proceeding

3D imaging of lung tissue by confocal microscopy and micro-CT

Andres Kriete, Andreas Breithecker and Wigbert D Rau
Proceedings of SPIE, v 4257(1), pp 469-476
09 Jul 2001

Abstract

Two complementary techniques for the imaging of tissue subunits are discussed. A computer guided light microscopic imaging technique is described first, which confocally resolves thick serial sections axially. The lateral area of interest is increased by scanning a mosaic of images in each plane. Subsequently, all images are fused digitally to form a highly resolved volume exhibiting the fine structure of complete respiratory units of lung. A different technique described is based on microtomography. This method allows to image volumes up to 3x3x3 cm at a resolution of up to 7 microns. Due to the lack of strong density differences, a contrast enhancement procedure is introduced which makes this technique applicable for the imaging of lung tissue. Imaging, visualization and analysis described here are parts of an ongoing project to model structure and to simulate function of tissue subunits and complete organs.

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Web of Science research areas
Engineering, Biomedical
Optics
Physics, Applied
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