Conference proceeding
A shear threshold for rolling adhesion of particles to bioreactive surfaces: influence of receptor and ligand site density
Proceedings of the IEEE 28th Annual Northeast Bioengineering Conference (IEEE Cat. No.02CH37342), v 2002-, pp 117-118
2002
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Abstract
The selectin family of cell adhesion molecules mediates capture and rolling adhesion of white blood cells to vascular walls, an essential component of the inflammatory response. Adhesion through L-selectin requires a hydrodynamic shear stress above a threshold level, a phenomenon known as the shear threshold effect. We have reported that the shear threshold effect can be re-created in cell-free systems, in which ligand-coated microspheres are perfused over L-selectin-coated surfaces. This paper extends the use of the cell-free system to determine the concurrent influence of L-selectin and ligand site density on the shear threshold effect. We find that the shear threshold effect diminishes with increasing levels of both L-selectin and its ligand. At reduced site densities of either L-selectin or ligand, the shear threshold effect is present, with maximal rolling observed at a shear stress of 1.2 dynes/cm/sup 2/. At higher site densities of L-selectin and ligand, the shear threshold effect disappears. These results suggest that a shear threshold is required for L-selectin-mediated rolling only when low numbers of receptor-ligand bonds can be formed. The appearance of the shear threshold effect may be controlled via receptor or ligand site density.
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Details
- Title
- A shear threshold for rolling adhesion of particles to bioreactive surfaces: influence of receptor and ligand site density
- Creators
- S.K. Bhatia - University of PennsylvaniaD.A. Hammer
- Publication Details
- Proceedings of the IEEE 28th Annual Northeast Bioengineering Conference (IEEE Cat. No.02CH37342), v 2002-, pp 117-118
- Publisher
- IEEE
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000175970000059
- Other Identifier
- 991021229895104721
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- Web of Science research areas
- Engineering, Biomedical