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Alginate strings acting as a directional gradient in spinal cord regeneration
Conference proceeding

Alginate strings acting as a directional gradient in spinal cord regeneration

A Orana, S Kanakasabai, R Murphy and M.A Wheatley
2003 IEEE 29th Annual Proceedings of Bioengineering Conference, pp 35-36
2003

Abstract

Adhesives Amino acids Antibiotics Biomedical engineering Chemical engineering Fibroblasts Genetic engineering Spinal cord Strontium Viscosity
We are designing a spinal cord graft that would aid in directionally specific neuronal regeneration. We plan to use genetically engineered cells that release neurotrophic factors ensheathed in modified alginate strings. Thus far, we have a method for making strings of alginate bioconjugated with a laminin pentapeptide (YIGSR), which support cell adhesion. These strings are 400-500 /spl mu/m in thickness. In vitro studies have shown that rat (NB2a) and human (SHSY5Y) neuroblastoma cell lines adhere to, and differentiate on these modified alginate strings. Currently we are aiming to establish a growth factor gradient to promote neuronal differentiation in a desired direction. These modified alginate strings, when surgically transplanted in the spinal cord, would set up a physical gradient providing directional regeneration of the adherent neuronal cells.

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Web of Science research areas
Computer Science, Artificial Intelligence
Engineering, Biomedical
Instruments & Instrumentation
Radiology, Nuclear Medicine & Medical Imaging
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