Conference proceeding
Application of digital image quality criteria to optimize the confocal microscope setup
Proceedings of SPIE, v 2184(1), pp 195-205
04 Apr 1994
Abstract
This paper discusses how digital image quality criteria help to optimize image quality, in particular for applications in laser scanning microscopy. Image quality considerations offer a uniform description of the available transfer characteristics, which are summed up and weighted properly to finally represent the system by a single number. In the spatial domain we can measure sharpness and contrast of the (digital) volumes by analyzing intensities and their local dependencies in a statistical fashion. This includes sum modulus difference, gray level variance, and lateral inhibition. Based on information theory, the criterion volume fidelity takes into account the knowledge of the spatial structure of a test object and compares the intensities with those present in the final digital image. Applications presented here include measurement of image quality improvement when going from non-confocal to confocal imaging, testing of new confocal system designs and the evaluation of digital post-processing methods. Limitations in the presence of noise are discussed.
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Details
- Title
- Application of digital image quality criteria to optimize the confocal microscope setup
- Creators
- Andres Kriete - Univ. Giessen (Germany)
- Publication Details
- Proceedings of SPIE, v 2184(1), pp 195-205
- Conference
- Three-Dimensional Microscopy: Image Acquisition and Processing
- Publisher
- Society of Photo-Optical Instrumentation Engineers (SPIE)
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:A1994BA45N00022
- Other Identifier
- 991019186670704721
InCites Highlights
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- Web of Science research areas
- Microscopy