Conference proceeding
Assessing Angiogenesis in Murine Glioma and Breast Tumor Models with Contrast Enhanced Ultrasound Imaging
2006 IEEE ULTRASONICS SYMPOSIUM, VOLS 1-5, PROCEEDINGS, v 1, pp 416-419
01 Jan 2006
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
The purpose of this study was to compare 4 contrast-enhanced ultrasound (US) techniques for measuring tumor neovascularity to 4 markers of angiogenesis in 2 xenograft rat models. Glioma (C6; 75 rats) or breast cancer (NMU; 72 rats) cells were implanted in 147 rats. The US contrast agent Optison (GE Healthcare, Princeton, NJ) was injected in a tail vein (dose: 0.4 ml/kg). Power Doppler imaging (PDI), pulse-subtraction harmonic imaging (PSHI), flash-echo imaging (FEI) and Microflow imaging (MFI) was performed with an Aplio scanner (Toshiba America Medical Systems, Tustin, CA) and a 7.5 MHz linear array. Digital US clips of the tumors were used with image-processing software to calculate fractional tumor neovascularity as contrast enhanced pixels over tumor area (for US) and staining over tumor area (for specimens). Results were compared using linear regression analysis and Z-tests. The tortuous morphology of tumor neovessels (i.e., irregular vessel sizes and distribution) was visualized better with MFI than with the other US modes. The strongest correlation found by linear regression in the C6 model was between PSHI and percent area stained with CD31 (r = 0.37, p < 0.0001). Significant correlation was also shown between the US techniques and bFGF, VEGF and CD31. In the NMU model the strongest correlation was between FEI and COX-2 (r = 0.46,p < 0.0001), but all the US methods and stains did correlate; albeit to a lesser degree (r > 0.22, p < 0.04). There were no statistically significant differences between correlations obtained with the various US methods (p > 0.15). In conclusion, Quantitative contrast-enhanced US measures of tumor neovascularity in the glioma and breast cancer xenograft models C6 and NMU appear to provide a noninvasive marker for angiogenesis.
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Details
- Title
- Assessing Angiogenesis in Murine Glioma and Breast Tumor Models with Contrast Enhanced Ultrasound Imaging
- Creators
- R. J. Ro - Thomas Jefferson UniversityF. Forsberg - Thomas Jefferson UniversityJ. B. Liu - Thomas Jefferson UniversityT. B. Fox - Thomas Jefferson UniversityM. Potoczek - Thomas Jefferson Univ, Radiat Oncol, Philadelphia, PA 19107 USAS. Y. Chiou - Thomas Jefferson Univ, Dept Radiol, Philadelphia, PA 19107 USAP. A. Lewin - Drexel Univ, Sch Biomed Engn, Philadelphia, PA 19107 USAB. B. Goldberg - Thomas Jefferson Univ, Dept Radiol, Philadelphia, PA 19107 USAIEEE
- Publication Details
- 2006 IEEE ULTRASONICS SYMPOSIUM, VOLS 1-5, PROCEEDINGS, v 1, pp 416-419
- Series
- Ultrasonics Symposium
- Publisher
- IEEE
- Number of pages
- 2
- Grant note
- CA93907 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Toshiba America Medical Systems GE Healthcare; General Electric
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems; Games, Artificial Intelligence, and Media Systems (GAIMS) Center
- Web of Science ID
- WOS:000260407800098
- Scopus ID
- 2-s2.0-46849108379
- Other Identifier
- 991019167638604721
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- Collaboration types
- Domestic collaboration
- Web of Science research areas
- Acoustics
- Engineering, Electrical & Electronic
- Physics, Applied