Conference proceeding
Characterization of Ultrasound-Triggered Bulk Antibiotic Release from Novel Spinal Hardware
2019 IEEE International Ultrasonics Symposium (IUS), v 2019-, pp 231-234
Oct 2019
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
This study evaluated the efficacy of ultrasound-triggered drug delivery devices against bacterial infection in an ex vivo cadaveric rabbit spine model. Polylactic acid (PLA)-coated, vancomycin (VAN)-loaded polyether ether ketone (PEEK) devices (1 cm 3 ) with a drug-loading reservoir (0.785 cm 3 ) were 3D printed. Two device designs were evaluated: 1 large hole for drug release vs 2 smaller holes. Clips were implanted medial to the spinal midline in mature (~6 months, 3 kg) female White New Zealand cadaveric rabbits (n=4) under an IACUC-approved protocol. To simulate infection, 10 4 CFU of Staphylococcus aureus were added to 2 of the 4 sites; the other 2 sites were left clean. Two of the 4 sites (1 inoculated, 1 clean) were insonated for 20 minutes with a Logiq E9 ultrasound scanner (GE Healthcare, Waukesha, WI, USA) equipped with a C1-6 curvilinear probe, using power Doppler imaging (1.7 MHz frequency, 6.4 kHz PRF, 100% acoustic output power) to induce rupture of the PLA coating for VAN release. Infected sites showed marked reduction in bacterial colonization following ultrasound-triggered VAN release, while uninsonated sites exhibited little reduction in bacterial colonization. At 48 hours, there was significantly greater VAN release from the insonated clips compared to the uninsonated clips (p <; 0.04). There was significantly greater ultrasound-triggered total VAN release from the 1-hole device design than from the 2-hole design (7420 ± 2992 μg vs. 3500 ± 954 μg, p <; 0.0001). These levels are sufficient to prevent adhesion of S. aureus to implant materials. This study demonstrated the feasibility of an ultrasound-mediated antibiotic delivery device, which could become a potent weapon against spinal surgical site infections.
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Details
- Title
- Characterization of Ultrasound-Triggered Bulk Antibiotic Release from Novel Spinal Hardware
- Creators
- Lauren J Delaney - Thomas Jefferson University,Department of Radiology,Philadelphia,PA,USANoreen J Hickok - Thomas Jefferson UniversityFlemming Forsberg - Thomas Jefferson UniversityAlex M Sevit - Drexel UniversityJohn R Eisenbrey - Thomas Jefferson UniversityKeith Fitzgerald - Thomas Jefferson UniversityChristopher K Kepler - Thomas Jefferson UniversityTaolin Fang - Thomas Jefferson UniversitySamantha L Knott - Thomas Jefferson UniversityPriscilla Machado - Thomas Jefferson UniversitySteven M Kurtz - Drexel UniversityIEEE
- Publication Details
- 2019 IEEE International Ultrasonics Symposium (IUS), v 2019-, pp 231-234
- Publisher
- IEEE
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000510220100060
- Scopus ID
- 2-s2.0-85077530913
- Other Identifier
- 991019170325404721
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- Web of Science research areas
- Engineering, Electrical & Electronic