Conference proceeding
Ethanol stimulates phosphoinositide-specific phospholipase C in rat hepatocytes
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol.45:6
01 May 1986
Abstract
Ethanol (50-300 mM) causes a transient mobilization of calcium from hormone-sensitive pools in rat hepatocytes. They have studied the action of ethanol on polyphosphoinositide and inositol phosphate levels in these cells. Addition of ethanol to isolated hepatocytes prelabelled with /sup 32/Pi resulted in a small (3-5%) decrease in the level of (/sup 32/P)-phosphatidylinositol 4,5-bisphosphate and a 10-15% increase in (/sup 32/P) phosphatidylinositol 4-phosphate and (/sup 32/P) phosphatidic acid. Maximal changes were seen within 30 sec after ethanol. (/sup 32/P) phosphatidylcholine was unaffected. The changes were concentration dependent over a range of 50-500 mM ethanol. Phorbol esters, which inhibit ethanol-induced calcium mobilization, also inhibited these effects of ethanol on polyphosphoinositides. In intact hepatocytes labeled with myo-(2-/sup 3/H)inositol a significant ethanol-induced increase in (/sup 3/H)inositol phosphates could not be detected. After permeabilization of the cells with digitonin, however, ethanol caused a time and concentration dependent release of (/sup 3/H) inositol trisphosphate and (/sup 3/H)inositol bisphosphate. These results indicate that ethanol-induced calcium mobilization in hepatocytes is due to the activation by ethanol of the phosphoinositide-specific phospholipase C.
Metrics
3 Record Views
Details
- Title
- Ethanol stimulates phosphoinositide-specific phospholipase C in rat hepatocytes
- Creators
- R RubinA ThomasJ.B HoekHahnemann Univ. School of Medicine, Philadelphia, PA
- Publication Details
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol.45:6
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- General Internal Medicine
- Identifiers
- 991019184016804721