Conference proceeding
Immobilization of Chondroitin Sulfate for the Fabrication of Biomimetic Brush Structures
2010 IEEE 36TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE
Annual IEEE Northeast Bioengineering Conference
01 Jan 2010
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Intervertebral disc (IVD) degeneration is accompanied by a loss of proteoglycans (PGs) in the nucleus pulposus (NP), resulting in a loss of charged chondroitin sulfate (CS) chains, decreasing mechanical function of the disc. PG mimics may be employed to restore NP functionality. Synthesis of CS bottle brush structures requires immobilization of CS at its terminal end. In this study, we have investigated commercially available CS for use in CS bottle brush synthesis. A primary amine (PA) at the terminal end of CS was identified and utilized to conjugate amine-reactive monomers to CS. Conjugation of monomers to CS was confirmed using the fluorescamine assay. Percent PA conjugations of 26%, 80% and 99% were seen for acrolein, acrylic acid and allyl glycidyl ether respectively. CS was also immobilized onto epoxy and aldehyde functionalized surfaces via the CS terminal primary amine. CS attachment was determined by monitoring changes in substrate sulfur content (energydispersive X-ray EDAX microanalysis) and surface wettability (contact angle). The modification and immobilization of CS demonstrated here will facilitate the synthesis of CS bottle brush PG mimics.
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Details
- Title
- Immobilization of Chondroitin Sulfate for the Fabrication of Biomimetic Brush Structures
- Creators
- S. Sarkar - Drexel Univ, Philadelphia, PA 19104 USAN. Ganesh - Drexel Univ, Philadelphia, PA 19104 USAC. L. Schauer - Drexel UniversityE. Vresilovic - Drexel Univ, Philadelphia, PA 19104 USAM. Marcolongo - Drexel Univ, Philadelphia, PA 19104 USAIEEE
- Publication Details
- 2010 IEEE 36TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE
- Series
- Annual IEEE Northeast Bioengineering Conference
- Publisher
- IEEE
- Number of pages
- 2
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Materials Science and Engineering
- Identifiers
- 991019170352304721
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- Web of Science research areas
- Engineering, Biomedical