Conference proceeding
Inducing intracellular ros and cellular redox without cell death in mesenchymal cells using microsecondpulsed DBD plasma
2012 Abstracts IEEE International Conference on Plasma Science, 7E-7
Jul 2012
Abstract
Intracellular reactive oxygen species (ROS) is a known activator of cell signaling, however too much ROS causes cell death. Our goal was to determine the optimal microsecond-pulsed Dielectric Barrier Discharge plasma (DBD-plasma) treatment dose (J/cm 2 ) in which ROS/redox responses would potentially activate cell function but not cell death. In this study, we applied DBD-plasma to mesenchymal cells and evaluated intracellular ROS generation, redox, mitochondrial membrane potential and the Live/Dead response to increasing levels of DBD-plasma. Using fluorescent indicators to measure superoxide anion (O 2 - .) (MitoSOX™ Red), reduced glutathione (GSH) (ThiolTracker™ Violet) and mitochondrial membrane potential (ΔΨm) (MitoTracker® Red), we show that DBD-plasma doses of 1-5 J/cm 2 stimulated an immediate (minutes) increase in O 2 - levels with a concomitant decrease in reduced thiol. The mitosox fluorescence intensity increased with DBD-plasma treatments until 1 J/cm 2 , after which levels plateaued regardless of increased dose. Reduced thiol fluorescent intensity showed an immediate 50% decrease regardless of DBD-plasma treatment dose. A 20% decrease in mitochondrial membrane potential occurred at 2 hrs post-DBD-plasma treatments 1 - 5 J/cm 2 , with significant cell death observed (fluorescent Live/Dead assay; syto- 13/propidium iodide) at levels of 2.5 J/cm 2 and greater. Taken together, these results provide a range in which DBD (0-2.0 J/cm 2 ) intracellular ROS generation and cellular redox change with little effect on cell viability. Understanding the optimal treatment levels DBD-plasma interactions is essential for developing DBD-plasma treatments to induce directed cell function such as; proliferation, differentiation and targeted gene expression.
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Details
- Title
- Inducing intracellular ros and cellular redox without cell death in mesenchymal cells using microsecondpulsed DBD plasma
- Creators
- N Shainsky - Drexel UniversityG Friedman - Drexel UniversityG Fridman - Drexel UniversityA Fridman - Drexel UniversityM. J Steinbeck - Drexel UniversityT. A Freema - Thomas Jefferson University
- Publication Details
- 2012 Abstracts IEEE International Conference on Plasma Science, 7E-7
- Publisher
- IEEE
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Electrical and Computer Engineering; Mechanical Engineering and Mechanics
- Other Identifier
- 991019182660204721