Conference proceeding
Nanoencapsulation of Organophosphorus Acid Anhydrolase with Mesoporous Materials for Chemical Agent Decontamination in Organic Solvents
ANTITERRORISM AND HOMELAND DEFENSE: POLYMERS AND MATERIALS, v 980, pp 233-252
01 Jan 2007
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Organophosphorus acid anhydrolase (OPAA) was successfully nanoencapsulated, in-situ, into both silica and organic-modified silica following the low volume shrinkage, non-surfactant templated sol-gel process with D-fructose and poly(ethylene glycol) as the pore-forming agents. By varying the concentration of the template or the concentration of the starting materials, the pore parameters were tuned to have high surface area of 500-800 m(2)/g, large pore volume from 0.2-0.8 cm(3)/g, and pore diameters ranging from 2-6 rim. As a result, the enzyme remained active in the nanoencapsulated form in both aqueous and mixed aqueous-organic solvents and was reusable by employing a simple regeneration procedure of buffer wash. The immobilization of OPAA in mesoporous materials significantly increased the stability of OPAA against the denaturation in the presence of organic solvents. For a remarkable example, the organically-modified gel sample in 20% acetone significantly retained enzyme activity up to similar to 90% in comparison with aqueous solution.
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Details
- Title
- Nanoencapsulation of Organophosphorus Acid Anhydrolase with Mesoporous Materials for Chemical Agent Decontamination in Organic Solvents
- Creators
- Kate K. Ong - Drexel UniversityTu-Chen ChengRay YinHua Dong - Drexel Univ, Dept Chem, Philadelphia, PA 19104 USAJian-Min YuanYen Wei - Drexel Univ, Dept Chem, Philadelphia, PA 19104 USA
- Contributors
- J G Reynolds (Editor)G E Lawson (Editor)C J Koester (Editor)
- Publication Details
- ANTITERRORISM AND HOMELAND DEFENSE: POLYMERS AND MATERIALS, v 980, pp 233-252
- Series
- ACS SYMPOSIUM SERIES
- Publisher
- American Chemical Society; Washington, DC
- Number of pages
- 20
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Physics
- Web of Science ID
- WOS:000269053300014
- Scopus ID
- 2-s2.0-51049103150
- Other Identifier
- 991019169101304721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Chemistry, Multidisciplinary
- Polymer Science