Conference proceeding
Prolonged exposure to morphine induces cell adhesion in an in vitro model of the blood-brain barrier
Vol.9(1), pp.41-42
20th Annual Conference of the Society on Neuroimmune Pharmacology (SNIP 2014), 20th (New Orleans, Louisiana, United States, 26 Mar 2014 - 29 Mar 2014)
2014
Abstract
Opioid abuse by human immunodeficiency virus type 1 (HIV-1)-infected individuals leads to more rapid disease progression, increased viral replication and peripheral viral load, and increased incidence and severity of neurocognitive abnormalities compared to non-drug abusers. The blood-brain barrier (BBB) is an obstacle that must be overcome during neuroinvasion with subsequent development of HIV-associated neurocognitive disorders (HAND). Previous studies of mu-opioids and alteration of BBB permeability have suggested that exposure increases cellular transmigration through an uncharacterized mechanism. In this study, a human brain microvascular endothelial cell (hBMEC) line, hCMEC/D3, was used to establish an in vitro transwell model of the BBB to investigate the effects of chronic (24, 48, 72 h) morphine treatment on barrier structure and function. We observed that hCMEC/D3 cells formed a confluent monolayer with a basal rate of passage of a tracer molecule comparable to primary hBMECs. It has also been shown that these cells express mu opioid receptor, and that prolonged morphine treatment induces changes in mRNA levels of cellular adhesion molecules. Functionally, an increase in PBMC transmigration and firm adhesion was observed following prolonged morphine exposure, in the absence of an increase in overall barrier leakiness. These results have suggested that morphine activates hCMEC/D3 cells leading to a cell environment permissive to transmigration. These studies may uncover a mechanism by which morphine disrupts periphery-CNS homeostasis leading to accelerated HAND. Supported by This work is supported by NIH/NINDS R01 NS32092, NIDA R01 DA19807, NIMH P30 MH092177, and NIMH T32 MH079785.
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Details
- Title
- Prolonged exposure to morphine induces cell adhesion in an in vitro model of the blood-brain barrier
- Creators
- Vanessa Pirrone - Microbiology and ImmunologyLin W - University of PennsylvaniaFeng R - University of PennsylvaniaWigdahl B - Drexel UniversityM R Nonnemacher - Drexel University
- Publication Details
- Vol.9(1), pp.41-42
- Conference
- 20th Annual Conference of the Society on Neuroimmune Pharmacology (SNIP 2014), 20th (New Orleans, Louisiana, United States, 26 Mar 2014 - 29 Mar 2014)
- Publisher
- Springer New York LLC
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Identifiers
- 991019170454404721