Conference proceeding
Quantification of beading intensity in cultured neurons
Bioengineering, 5778566
01 Apr 2011
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
We present an implemented method for automated quantification of neuronal beading. Beading is the "morphological hallmark" of axonal injury and is present in many neurological disease conditions as well. In vitro models of injury result in beading which has previously been difficult to quantify, and therefore difficult to use as a measurement of the degree of axonal injury. The beading program was designed to quantify beading intensity in single axons and output a unitless beading number that represents the size and number of beads in relation to the length and width of the axon. The program was used to analyze a sample set of images and was successful in demonstrating a quantifiable difference between injured neurons and sham controls. Bland-Altman analysis was used to assess agreement between the number of beads counted by the program and those counted manually.
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Details
- Title
- Quantification of beading intensity in cultured neurons
- Creators
- R. M. Dastgheyb - Drexel UniversityG. Gallo - Drexel UniversityK. A. Barbee - Drexel University
- Publication Details
- Bioengineering, 5778566
- Conference
- 2011 IEEE 37th Annual Northeast Bioengineering Conference (NEBEC) (Troy, NY, USA, 01 Apr 2011–03 Apr 2011)
- Series
- Annual IEEE Northeast Bioengineering Conference
- Publisher
- IEEE
- Number of pages
- 2
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems
- Web of Science ID
- WOS:000297284900049
- Scopus ID
- 2-s2.0-79958731424
- Other Identifier
- 991019170158804721
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InCites Highlights
Data related to this publication, from InCites Benchmarking & Analytics tool:
- Web of Science research areas
- Cell & Tissue Engineering
- Engineering, Biomedical
- Materials Science, Biomaterials