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Conference proceeding
Rolling adhesion of yeast engineered to express cell adhesion molecules
Proceedings of the IEEE 28th Annual Northeast Bioengineering Conference (IEEE Cat. No.02CH37342), v 2002-, 999492
2002
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Abstract
Selectins are cell adhesion molecules that mediate capture of leukocytes on vascular endothelium as an essential component of the inflammatory response. Here we describe a method for yeast surface display of selectins, together with a functional assay that measures rolling adhesion of selectin-expressing yeast on a ligand-coated surface. E-selectin-expressing yeast roll specifically on surfaces bearing sialyl-Lewis/sup x/ ligands. Observation of yeast rolling dynamics at various stages of their life cycle indicates that the kinematics of yeast motion depends on the ratio of the bud radius to the parent radius (B/P). Large-budded yeast "walk" across the surface, alternately pivoting about bud and parent. Small-budded yeast "wobble" across the surface, with bud pivoting about parent. Tracking the bud location of budding yeast allows measurement of the angular velocity of the yeast particle. Comparison of translational and angular velocities of budding yeast demonstrates that selectin-expressing cells are rolling rather than slipping across ligand-coated surfaces.
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Details
- Title
- Rolling adhesion of yeast engineered to express cell adhesion molecules
- Creators
- S.K. Bhatia - University of PennsylvaniaJ.S. Swers - University of PennsylvaniaR.T. CamphausenK.D. Wittrup - University of PennsylvaniaD.A. Hammer
- Publication Details
- Proceedings of the IEEE 28th Annual Northeast Bioengineering Conference (IEEE Cat. No.02CH37342), v 2002-, 999492
- Publisher
- IEEE
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Microbiology and Immunology
- Web of Science ID
- WOS:000175970000058
- Scopus ID
- 2-s2.0-0036079517
- Other Identifier
- 991021229905104721
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- Web of Science research areas
- Engineering, Biomedical