Conference proceeding
Three-dimensional Liver Cell Cultures Enhance Urea Synthesis and Drug Metabolism
2010 IEEE 36TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE
Annual IEEE Northeast Bioengineering Conference
01 Jan 2010
Featured in Collection : UN Sustainable Development Goals @ Drexel
Abstract
Tissue engineering within three-dimensional scaffolds represents a developing field with the potential to create in vitro livers. Human liver cells, HepG2, respond to cues in their immediate microenvironment by modulating cell functions including cell proliferation, urea synthesis and drug metabolism. The importance of three-dimensional environments draws from the fact that it has been extensively used for guided tissue regeneration. HepG2 cells grown in traditional two-dimensional supports were compared to three-dimensional cultures in polystyrene scaffolds. The 5-day experimental study examined the effects of the different environments on HepG2 cell urea synthesis using a quantitative colorimetric assay and drug metabolism-specific function using the cytochrome P450 monooxygenase substrate, 7-ethoxy-4-trifluoromethyl-coumarin. The function of liver cells grown on three-dimensional scaffolds was enhanced compared to the activity of cells grown on two-dimensional supports. The results suggest that HepG2 liver cell function in three-dimensional environments more closely mimic physiological responses than existing two-dimensional culture systems.
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Details
- Title
- Three-dimensional Liver Cell Cultures Enhance Urea Synthesis and Drug Metabolism
- Creators
- Dheeraj Roy - Drexel Univ, Philadelphia, PA 19104 USAWei Sun - Drexel UniversityIEEE
- Publication Details
- 2010 IEEE 36TH ANNUAL NORTHEAST BIOENGINEERING CONFERENCE
- Series
- Annual IEEE Northeast Bioengineering Conference
- Publisher
- IEEE
- Number of pages
- 2
- Resource Type
- Conference proceeding
- Language
- English
- Academic Unit
- Mechanical Engineering and Mechanics
- Identifiers
- 991019170576304721
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- Web of Science research areas
- Engineering, Biomedical