Dataset
Assessing the Effect of Exercise on the Expression of Genes Associated with Macrophage Activation State and Cytokines in the Dorsal Root Ganglia after C5 Unilateral Contusion in Female Sprague Dawley Rats
2025
Abstract
STUDY PURPOSE: Neuropathic pain is a common secondary complication that develops following a spinal cord injury (SCI). Previous works have shown that physical exercise initiated in the acute, but not chronic stages after SCI can alter macrophage presence in the dorsal root ganglia (DRG) and prevent neuropathic pain development. This study aims to further investigate how macrophage activity evolves over time after SCI, and how this activity is modulated by short bouts of aerobic or strength training initiated at acute, subacute, or chronic stages of spinal cord injury. DATA COLLECTED: Adult (age > 6 weeks), female Sprague Dawley rats received a C5 unilateral contusion SCI. Beginning 1-, 2-, or 5- weeks post-injury, SCI rats underwent 5 consecutive days of either aerobic training via 20 minutes of forced wheel running or strength training via 50 repetitions of an isometric lever pulling task. Following the last exercise session, C7-C8 ipsilesional DRGs were harvested, and ribonucleic acid (RNA) was extracted from these tissues for real-time polymerase chain reaction (RT-qPCR) analysis for the expression of genes associated with macrophage polarization state and cytokine production. DATA USAGE NOTES:
Metrics
25 Record Views
Details
- Title
- Assessing the Effect of Exercise on the Expression of Genes Associated with Macrophage Activation State and Cytokines in the Dorsal Root Ganglia after C5 Unilateral Contusion in Female Sprague Dawley Rats
- Creators
- Jason Wheeler - Department of Neurobioloyg & Anatomy, Marion Murray Spinal Cord Research Center, College of Medicine, Drexel UniversityMegan Detloff - Department of Neurobioloyg & Anatomy, Marion Murray Spinal Cord Research Center, College of Medicine, Drexel University
- Publisher
- Open Data Commons for Spinal Cord Injury (ODC-SCI)
- Grant note
- Craig H. Neilsen Foundation (#457508 (MRD)) NIH NINDS (NS097880 (MRD)) NIH NINDS (T32 NS121768 (JJW))
- Resource Type
- Dataset
- Language
- English
- Academic Unit
- Neurobiology and Anatomy
- Other Identifier
- 991022064981904721