Dataset
DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation
16 Oct 2020
Abstract
Biomaterials can improve the safety and presentation of therapeutic agents for effective immunotherapy, and a high level of control over surface functionalization is essential for immune cell modulation. Here, we developed biocompatible immune cell engaging particles (ICEp) that use synthetic short DNA as scaffolds for efficient and tunable protein loading. To improve the safety of chimeric antigen receptor (CAR) T cell therapies, micron-sized ICEp were injected intratumorally to present a priming signal for systemically administered AND-gate CAR-T cells. Locally retained ICEp presenting a high density of priming antigens activated CAR-T cells, driving local tumor clearance while sparing uninjected tumors in immunodeficient mice. The ratiometric control of costimulatory ligands (anti-CD3 and anti-CD28 antibodies) and the surface presentation of a cytokine (IL-2) on ICEp were shown to significantly impact human primary T cell activation phenotypes. This modular and versatile biomaterial functionalization platform can provide new opportunities for immunotherapies.
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Details
- Title
- DNA scaffolds enable efficient and tunable functionalization of biomaterials for immune cell modulation
- Creators
- Xiao Huang - University of California, San FranciscoJasper Z. Williams - University of California, San FranciscoRyan Chang - University of California, San FranciscoZhongbo Li - University of California, San FranciscoCassandra E. Burnett - University of California, San FranciscoRogelio Hernandez-Lopez - University of California, San FranciscoInitha Setiady - University of California, San FranciscoEric Gai - University of California, San FranciscoDavid M. Patterson - University of California, San FranciscoWei Yu - University of California, San FranciscoKole T. Roybal - University of California, San FranciscoWendell A. Lim - University of California, San FranciscoTejal A. Desai - University of California, San Francisco
- Publisher
- Dryad
- Grant note
- 1U54CA244438 / National Cancer Institute (https://ror.org/040gcmg81)
- Resource Type
- Dataset
- Language
- English
- Academic Unit
- School of Biomedical Engineering and Science
- Other Identifier
- 991022130763604721