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Data from: Fine mapping quanitative trait loci affecting murine external ear tissue regeneration in the LG/J by SM/J advanced intercross line
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Data from: Fine mapping quanitative trait loci affecting murine external ear tissue regeneration in the LG/J by SM/J advanced intercross line

Ellen Heber-Katz, James M. Cheverud, Heather A. Lawson, Kristine Bouckaert, Elizabeth P. Blankenhorn, Laura Cort, Andrew V. Kossenkov, Louise C. Showe, Khamilia Bedelbaeva, Dmitri Gourevitch, …
15 Nov 2013
DOI: https://doi.org/10.5061/dryad.vc473
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https://doi.org/10.5061/dryad.vc473View
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Abstract

LG/J QTL SM/J tissue regeneration Wound healing
External ear hole closure in LG/J mice represents a model of regenerative response. It is accompanied by the formation of a blastema-like structure and the re-growth of multiple tissues including cartilage. The ability to regenerate tissue is heritable. An F34 advanced intercross line of mice (Wustl:LG,SM-G34) was generated to identify genomic loci involved in ear hole closure over a 30 day healing period. We mapped nineteen quantitative trait loci (QTL) for ear hole closure. Individual gene effects are relatively small (0.08 mm) and most loci have co-dominant effects with phenotypically intermediate heterozygotes. QTL support regions were limited to a median size of 2 Mb containing a median of 19 genes. Positional candidate genes were evaluated using differential transcript expression between LG/J and SM/J healing tissue, function analysis, and bioinformatic analysis of SNPs in and around positional candidate genes of interest. Analysis of the set of 34 positional candidate genes and those displaying expression differences revealed over-representation of genes involved in cell cycle regulation/DNA damage, cell migration and adhesion, developmentally related genes, and metabolism. This indicates that the healing phenotype in LG/J mice involves multiple physiological mechanisms.

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