Files and links (1)
PDFOpen Access (License Unspecified), Open Access
Dissertation
Cdc42EP3-bound septin filaments promote actin filament assembly
Doctor of Philosophy (Ph.D.), Drexel University
Aug 2023
DOI:
https://doi.org/10.17918/00001829
Abstract
Septins are filament-forming proteins that are involved in numerous cellular processes. Septins co-localize with actin, microtubule and membrane structures. The Cdc42 effector protein Cdc42EP3 (also known as BORG2) regulates septin localization to cellular actin structures, but it is unknown how Cdc42EP3 controls septin-actin association. Using biochemical analysis with purified components, I show that Cdc42EP3 binds to both septins and actin in a Cdc42-regulated fashion. Importantly, septin-bound Cdc42EP3 accelerates actin filament polymerization. Septin filaments composed of SEPT2, SEPT6 and SEPT7 directly interact with actin filaments, although this occurs less efficiently in pre-formed filaments. Thus, Cdc42EP3 is not needed to recruit actin filaments to septin filaments at equilibrium. Instead, Cdc42EP3 recruits actin monomers to septin filaments, promoting localized actin filament formation and septin-actin structure assembly.
Metrics
45 File views/ downloads
13 Record Views
Details
- Title
- Cdc42EP3-bound septin filaments promote actin filament assembly
- Creators
- Meagan Tomasso
- Contributors
- Shae B. Padrick (Advisor)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- xiii, 219 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Biochemistry and Molecular Biology; College of Medicine; Drexel University
- Other Identifier
- 991021229715504721