Dissertation
Cell surface envelope protein transformation and membrane disruption by HIV-1 entry inhibitors
Doctor of Philosophy (Ph.D.), Drexel University
Jun 2020
DOI:
https://doi.org/10.17918/00000025
Abstract
This work examined transformations of the HIV-1 fusion protein Env and its anchoring membrane that can be subverted by lytic entry inhibitors to inactivate Env on viruses and cells. Two entry inhibitors were investigated: KR13 and M*DAVEI. KR13 is a chemically synthesized peptide triazole thiol (PTT) comprised of a CD4 binding site pharmacophore linked to a disulfide-targeting thiol, while M*DAVEI is a chimeric recombinant protein containing a gp120-glycan-binding lectin linked to Trp3, a gp41-binding fragment of Env's membrane proximal external region (MPER). Both inhibitors have previously been shown to cause virus lysis, indicating that they can trigger membrane disruption. Env-presenting cells were selected for these experiments to facilitate mechanistic studies and provide preliminary data for further cell-based studies.
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Details
- Title
- Cell surface envelope protein transformation and membrane disruption by HIV-1 entry inhibitors
- Creators
- Charles Gotuaco Ang - Drexel University, Biochemistry and Molecular Biology
- Contributors
- Irwin Chaiken (Advisor)Kenneth A. Barbee (Advisor)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- xvii, 175 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- School of Biomedical Engineering, Science, and Health Systems (1997-2026); Drexel University
- Other Identifier
- 991014695235904721