Files and links (1)
PDFOpen Access (License Unspecified), Open Access
Dissertation
Design and evaluation of polymeric chemoattractants for human melanoma cells
Doctor of Philosophy (Ph.D.), Drexel University
Jun 1994
DOI:
https://doi.org/10.17918/00000621
Abstract
Liquid phase photografting of vitamin K-1 and hydroquinone (HQ) were carried out onto medical grade polypropylene (PP) films with the aid of benzophenone (BP), and the effects of surface properties on transformed CHO-K1 cell adhesion as well as on the migrational behavior of human melanoma cells were investigated. The effects of various reaction conditions and the changes in physicochemical properties of the surface of PP were determined by EA, FMIR, and surface tension measurements. The addition reaction of polypropylene to the unsaturated double bonds in vitamin K-1 were studied using FTIR and FTNMR. The free radical content of the samples due to enzymes redox reactions were examined by ESR. As BP concentration was increased, the graft yields for the VK-1-g-PP and HQ-g-PP reaction were increased up to specific point and then decreased. Graft yield was increased with irradiation time and dose concentration and then leveled off. The surface tension of samples increased with the increase in graft yield. The free radical content of the samples due to enzymes redox reactions were examined by ESR. The adhesion of CHO-K1 cells on the surfaces of samples was increased with the increase in graft yield of the samples. Primary and metastatic human melanoma cells showed an enhanced migrational behavior toward the prepared samples in this study.
Metrics
16 File views/ downloads
15 Record Views
Details
- Title
- Design and evaluation of polymeric chemoattractants for human melanoma cells
- Creators
- Heung Jae Chun
- Contributors
- Richard B. Beard (Advisor)
- Awarding Institution
- Drexel University
- Degree Awarded
- Doctor of Philosophy (Ph.D.)
- Publisher
- Drexel University; Philadelphia, Pennsylvania
- Number of pages
- xi, 131 pages
- Resource Type
- Dissertation
- Language
- English
- Academic Unit
- Biomedical Engineering and Science Institute (1970-1997); Drexel University
- Other Identifier
- 991014970190204721